Convergent trafficking pattern of leptin after endocytosis mediated by ObRa–ObRd
Identifieur interne : 001B68 ( Main/Exploration ); précédent : 001B67; suivant : 001B69Convergent trafficking pattern of leptin after endocytosis mediated by ObRa–ObRd
Auteurs : Hong Tu [États-Unis, République populaire de Chine] ; Weihong Pan [États-Unis] ; Laura Feucht [États-Unis] ; Abba J. Kastin [États-Unis]Source :
- Journal of Cellular Physiology [ 0021-9541 ] ; 2007-07.
English descriptors
- Teeft :
- Acid precipitation, Adipose tissue, Assay, Biochem biophys, Biol, Biological functions, Bjorbaek, Cells overexpressing, Cells overexpressing obra, Cellular physiology, Cerebral microvessels, Chloroquine, Choroid plexus, Degradation, Endocytosed, Endocytosed leptin, Endocytosis, Endothelial cells, Exocytosis, Exocytosis medium, Intact leptin, Intracellular, Intracellular degradation, Isoforms, Kastin, Leptin, Leptin receptor, Leptin receptor isoforms, Leptin transport, Long form, Lower level, Lysosomal, Lysosome, Mrna, Obra, Obrb, Obrc, Obrd, Overexpressing, Overexpression, Pathway, Peptide, Proteasome, Proteasome subunit, Proteasomes, Receptor, Receptor isoforms, Residual cell surface binding, Short form, Short forms, Short isoforms, Transfection, Transient transfection, Western blot analysis.
Abstract
The cellular effects of leptin are dependent on the receptor subtypes that mediate the signaling and fate of endocytosed leptin inside the cells. In this study, we examined the differences in receptor expression, endocytosis, intracellular degradation, and exocytosis of a trace amount of leptin in cells overexpressing ObRb and short forms of the leptin receptor. The relative contribution of proteasomes and lysosomes in the intracellular fate of leptin was also determined. There were three unusual findings: (1) all receptor subtypes could mediate the binding and endocytosis of leptin, although ObRb was expressed at a lower level than ObRa, ObRc, and ObRd after transient transfection. This indicates that ObRb can be a transporting receptor. (2) Once internalized, the intracellular degradation pattern and exocytosis of leptin were independent of the receptor subtype. (3) Endocytosed leptin could remain intact for at least 1 h. This stability was further enhanced by inhibition of lysosomal activity. Thus, the intracellular pool of intact leptin may allow prolonged biological functions for this adipokine. J. Cell. Physiol. 212: 215–222, 2007. © 2007 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jcp.21020
Affiliations:
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In this study, we examined the differences in receptor expression, endocytosis, intracellular degradation, and exocytosis of a trace amount of leptin in cells overexpressing ObRb and short forms of the leptin receptor. The relative contribution of proteasomes and lysosomes in the intracellular fate of leptin was also determined. There were three unusual findings: (1) all receptor subtypes could mediate the binding and endocytosis of leptin, although ObRb was expressed at a lower level than ObRa, ObRc, and ObRd after transient transfection. This indicates that ObRb can be a transporting receptor. (2) Once internalized, the intracellular degradation pattern and exocytosis of leptin were independent of the receptor subtype. (3) Endocytosed leptin could remain intact for at least 1 h. This stability was further enhanced by inhibition of lysosomal activity. Thus, the intracellular pool of intact leptin may allow prolonged biological functions for this adipokine. J. Cell. Physiol. 212: 215–222, 2007. © 2007 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Louisiane</li></region></list><tree><country name="États-Unis"><region name="Louisiane"><name sortKey="Tu, Hong" sort="Tu, Hong" uniqKey="Tu H" first="Hong" last="Tu">Hong Tu</name></region><name sortKey="Feucht, Laura" sort="Feucht, Laura" uniqKey="Feucht L" first="Laura" last="Feucht">Laura Feucht</name><name sortKey="Kastin, Abba J" sort="Kastin, Abba J" uniqKey="Kastin A" first="Abba J." last="Kastin">Abba J. Kastin</name><name sortKey="Pan, Weihong" sort="Pan, Weihong" uniqKey="Pan W" first="Weihong" last="Pan">Weihong Pan</name><name sortKey="Pan, Weihong" sort="Pan, Weihong" uniqKey="Pan W" first="Weihong" last="Pan">Weihong Pan</name><name sortKey="Pan, Weihong" sort="Pan, Weihong" uniqKey="Pan W" first="Weihong" last="Pan">Weihong Pan</name></country><country name="République populaire de Chine"><noRegion><name sortKey="Tu, Hong" sort="Tu, Hong" uniqKey="Tu H" first="Hong" last="Tu">Hong Tu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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